component 1: Orientation of pictures in the MicroscopePart 2: exercise with Depth of field in the MicroscopePart 3: investigation of Pond Water & microbe

(Adapted native

Parts that the Microscope:



Your microscope has 4 target lenses: Scanning (4x), low (10x), High (40x), and also Oil Immersion (100x). In this lab, you will certainly not use the oil immersion lens; that is for viewing microorganisms and requires technical instructions not covered in this procedure.

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In enhancement to the objective lenses, the ocular lens (eyepiece) has actually a magnification. The total magnification is figured out by multiply the magnification that the ocular and objective lenses.

Magnification Ocular Lens Total Magnification
Scanning 4x 10x 40x
Low Power 10x 10x 100x
High Power 40x 10x 400x
Oil Immersion 100x 10x 1000x

General Procedures:

1. Make certain all backpacks, purses, etc. Are off the benchtop.

2. Lug the microscope by the base and arm v both hands.

3. Store with the cord wrapped about the microscope and also the scanning target clicked right into place.

Focusing Specimens:

1. Plug your microscope into the strength supply and switch top top the illuminator.

2. Constantly start through the phase as low as possible and making use of scanning target (4x). Odds are, friend will have the ability to see miscellaneous on this setting (sometimes it’s only a color). Use the coarse knob to focus: the photo may be tiny at this magnification, but you won"t have the ability to find that on the greater powers without this first step. Move the mechanical phase until your concentrated image is additionally centered.

3. Once you"ve concentrated using the scanning objective, switch to the low power objective (10x). Use the outlet knob to refocus and also move the mechanical stage to re-center your image. Again, if you haven"t concentrated on this level, you will not be able to move to the next level.

4. Now switch come the high power objective (40x). In ~ this point, just use the fine adjustment knob to emphasis specimens.

5. If the specimen is as well light or too dark, try adjusting the diaphragm.


1. Store microscope with the scanning target in place and the stage in its lowest position.

2. Pave cords roughly the microscope.

3. Replace slides to initial slide tray.


Compound microscope Microscope slide with the letter “e”


Compound microscope Microscope slide with 3 threads


Compound microscope Microscope on slide Coverslip carry pipette Pond water sample


1. Utilizing the deliver pipette, transfer a autumn of pond water top top a microscope slide. The finest specimens typically come native the bottom and probably will contain chunks of birds or various other debris the you have the right to see through your nude eye.

2. Location the coverslip top top the slide.

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3. Usage the SCANNING (4x) objective to focus, then move the mechanical stage around to scan the slide for live microorganisms. Girlfriend are in search of tiny swim beings- they may look eco-friendly or clear and might be really small. Select one to focus on and also center that in her visual field.


You may wish to usage the ProtoSlo to store your organisms from swimming too quickly!