Explanation:

DNA helicase unwinds the dual helix, separating the 2 strands so they might be replicated by DNA polymerase.

You are watching: Which enzyme is responsible for unzipping the dna double helix

Primase to add an RNA inside wall to help initiate DNA replication. DNA ligase is responsible because that joining Okazaki fragments on the lagging strand during replication.


Explanation:

DNA replication is the process of copy the parental DNA helix into two similar daughter helices. The procedure is semi-conservative, which way that one parent strand is passed under to each daughter strand. The process begins as soon as helicase unwinds the twin helix and separates the two strands to create the replication fork. Topoisomerase helps this process by relieving rotational stress, overload on the helix as soon as it is gift unwound. DNA polymerase adds brand-new nucleotides to the daughter strand, manufacturing the new DNA strand.

During replication there is a leading strand, which occurs when replication occurs from 5" to 3" and also moves towards the replication fork, and a lagging strand, once replication occurs far from the replication fork. Replication occurs in brief segments top top the lagging strand, well-known as Okazaki fragments. The protein DNA ligase is responsible for finally fusing these pieces together after they room made by DNA polymerase.


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Example question #3 : Dna Replication


During DNA replication, one enzyme dubbed DNA helicase "unzips" the molecule the double-stranded DNA. What is the most likely mechanism that DNA helicase?


Possible Answers:

DNA helicase breaks down the hydrogen bonds in between purines and also purines


DNA helicase breaks under the hydrogen bonds in between the purines and pyrimidines


DNA helicase breaks down the covalent bonds between the purines and pyrimidines


DNA helicase breaks under the covalent bonds in between pyrimidines and pyrimidines


Correct answer:

DNA helicase breaks down the hydrogen bonds in between the purines and pyrimidines


Explanation:

The inquiry states that DNA helicase "unzips" the two strands the DNA; therefore, this enzyme should be breaking under the bonds in between base pairs.

The bonds between base pairs are dubbed hydrogen bonds, which is a noncovalent bond. This means that the DNA helicase is breaking down the hydrogen bonds between base bag in order to separate the 2 strands. In DNA, there room two type of base pairs: purines and also pyrimidines. Recall the adenine and also guanine room classified together purines vice versa, thymine and also cytosine are classified as pyrimidines; therefore, a base pairing in DNA constantly occurs in between a purine and a pyrimidine. This means that the DNA helicase is breaking down the hydrogen bonds between purines and also pyrimidines.


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Example concern #4 : Dna Replication


Of the complying with DNA replication proteins, i beg your pardon one web links the Okazaki fragments of the lagging strand?


Possible Answers:

Single-strand binding protein (SSB)


Helicase


DNA ligase


DNA polymerase III


DNA polymerase I


Correct answer:

DNA ligase


Explanation:

DNA ligase is the protein responsible for linking, or ligating, Okazaki fragments together in bespeak to type a single complete DNA strand. This action only essential on the lagging strand; the top strand can be made consistently by DNA polymerase due to the fact that it is may be to check out away from the replication fork in the 3"-to5" direction. Because the DNA polymerase ~ above the lagging strand have to read towards the replication form, it cannot by synthesized continuously.


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Example concern #5 : Dna Replication


DNA is naturally uncovered as a double-helix, yet for it to replicated that must very first be unwound so that DNA replication proteins can accessibility the 2 strands. The double-helix framework of DNA is very stable, and also after gift unwound for DNA replication to occur, the 2 strands can quickly return come the double-helix structure. If the strands re-anneal, proteins necessary for DNA replication can not enter and also begin the process of replication.


Which the the complying with pairs the DNA replication proteins is responsible because that unwinding the DNA double-helix and also maintaining the separation the the DNA strands?


Possible Answers:

Single-strand binding protein (SSB) and helicase


DNA polymerase and also DNA ligase


DNA ligase and helicase


DNA polymerase and also helicase


Helicase and single-strand binding protein (SSB)


Correct answer:

Helicase and single-strand binding protein (SSB)


Explanation:

Helicase is the protein resposible because that unwinding the DNA double-helix. Single-strand binding proteins affix to the freshly unwound strands that DNA and ensure that the strands perform not re-anneal. Helicase create the replication fork opening, permitting replication protein to enter and bind; single-strand binding proteins keep the replication fork open as proteins enter.


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Example concern #1 : Dna Replication


Which the the following causes a DNA fragment come be created in the 5" come 3" direction?


Possible Answers:

None the the choices


Polarity causes DNA polymerase III have the right to only add nucleotides to the 5" end of a DNA strand


Hydrogen bonds avoid DNA polymerase III from including nucleotides to a DNA strand


Polarity causes DNA polymerase III can only add nucleotides to the 3" finish of a DNA strand.


DNA polymerase III can add nucleotides to any type of random allude on a DNA strand regardless of polarity


Correct answer:

Polarity causes DNA polymerase III deserve to only add nucleotides to the 3" finish of a DNA strand.


Explanation:

A DNA fragment will certainly be developed in the 5" come 3" direction due to the fact that of the polarity of the DNA molecule. Including nucleotides come the 3" end enables DNA polymerase to use the phosphate molecules as "fuel," and include a new nucleotide come the DNA strand.


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Example question #1 : Dna


Which protein is responsible for the removal of the RNA inside wall from the 5" finish of a lagging strand of DNA, and replacing it v DNA nucleotides?


Possible Answers:

Primase


None of the choices


Topoisomerase


DNA Polymerase I


DNA Polymerase III


Correct answer:

DNA Polymerase I


Explanation:

DNA Polymerase I clears the primer from the 5" finish of a lagging strand, and also replaces it with DNA nucleotides. This permits DNA synthesis to begin on the lagging strand. 


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Example question #1 : Dna Replication


Which the the following RNA molecules is responsible for transporting the code that will certainly be check out at the ribosomes in stimulate to produce a protein? 


Possible Answers:

mRNA


snRNA


tRNA


rRNA


Correct answer:

mRNA


Explanation:

Messenger RNA, or mRNA, is the RNA strand the is transcribed indigenous the gene uncovered on DNA. The is responsible for being read by a ribosom in order to produce a protein.

Ribosomal RNA (rRNA) develops a structural ingredient of the ribosomes. Transfer RNA (tRNA) dead amino acid residues and provides one anticodon to include the amino acids to the growing protein at the ribosome. Small nuclear RNA (snRNA) are discovered in the nucleus and help regulate transcription and maintain telomere length.


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Example inquiry #1 : Dna Replication


DNA replication is semi-conservative. This means that __________.


Possible Answers:

an entirely new synthesized DNA molecule is created, when the original double helix remains together


parts from each initial strand will be supplied as templates when producing the new double helix, bring about a patchwork mix of both original and newly-synthesized nucleotides


both twin strands have various percentages the nucleotides


both dual strands have a newly created strand and an initial template strand


Correct answer:

both dual strands have a newly produced strand and an original template strand


Explanation:

DNA replication entails the separation the the two original DNA strands. Both of this strands room then replicated making use of DNA polymerase. This results in two DNA dual helices, each with a brand-new strand and an initial strand.

Consider this example, in i beg your pardon the parental strands are stood for by "P" and also the daughter strands are stood for by "D."

Before replication there room two parental strands: PP

The parental strands are split: p P

Daughter strands are created each parental strand: PDDP

The fully-replicated dual strands separate: PD DP

Each final strand has one parental strand (old DNA) and one daughter strand (new DNA).


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Example concern #1 : Dna Replication


The procedure of DNA replication is thought about semiconservative. DNA is produced by using one more DNA strand together a template, and also building a brand-new complementary strand onto the pre-existing strand. Each new DNA molecule includes one strand indigenous the parental template, and also one recently synthesized strand.


A solitary DNA molecule (one double-helix) undergoes three rounds the replication. After ~ the last replication is complete, how countless of the DNA molecules current do not contain any component of the initial template?


Possible Answers:

Two


Eight


Four


Six


Zero


Correct answer:

Six


Explanation:

O=Original strand, N=New strand

Before any replications: OO

After one ring of replication, both brand-new double-helices contain one strand from the original double-helix and also one freshly synthesized strand.

1 replication: ON1, N1O

After the second replication, there are now 4 double-helix molecules. 2 contain original strands in mix with new strands, and two contain only brand-new strands.

2 replications: ON2, N2N1, N1N2, N2O

After the thrid replication occasion there will certainly be eight total molecules. That these, 6 will contain only new strands and also two will contain a combination of original and brand-new strands.

3 replications: ON3, N3N2, N2N3, N3N1, N1N3, N3N2, N2N3, N3O

There must always be two double-helices that contain original strands, as there are always only two initial strands and they do not disappear.


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